First insight into how stress exposure triggers Vibrio harveyi recipient successful conjugation

Conjugation is the most common horizontal gene transfer (HGT) process that can be affected by environmental change and promote bacterial virulence drug resistance. However, it unknown whether changes influence conjugation ability of marine fish pathogen Vibrio harveyi , thereby affecting its pathogenicity This study systematically analyzes effect stress on V. to obtain shuttle plasmids from Escherichia coli during conjugation. The results indicate cannot receive plasmid pMMB207 without exposure stress. certain (37–46°C, 4%–16% ethanol, 0.14–0.56 mM SDS, 0.04~0.05 M NaOH, 0.012–0.024 HCl for 5–60 minutes) in log phase before successfully induces fertility recipient intergeneric mating with E. . In particular, ethanol heat showed strong induction up 2.5 × 10 5 5.3 3 transconjugants when exposed 16% minutes 40°C 60 minutes, respectively. Additionally, appropriate levels NaOH (0.05 M, minutes), SDS (0.42 mM, (0.024 lead 2.3 4.5 2 1.8 transconjugants, These will help establish homologous recombination knockout technology greatly advance molecular theoretical research They also support establishment disease prevention control strategies based interruption HGT regulation.